Composite

Part:BBa_K607002

Designed by: Olesja Popow   Group: iGEM11_Groningen   (2011-09-13)

PBad_LasR-LVA

lasR protein with LVA-tag under transcriptional control of the PBad Promoter. For more information about the pBAD/araC promotor, see: https://parts.igem.org/wiki/index.php?title=Part:BBa_K607001
The lasR gene has been discovered in Pseudomonas Aeruginosa contributing to its pathogenicity(1) and is also a quorum sensing regulator.(2) LasR-LVA is also a transcriptional activator of the elastase expression (1) and in our system, the LasR will also have a ssRA tag.

References:
(1) M J Gambello, and B H Iglewski. Cloning and characterization of the Pseudomonas aeruginosa lasR gene, a transcriptional activator of elastase expression. J Bacteriol. 1991.
(2) Mark E. Shirtliff, Jon T. Mader, and Anne K. Camper. Molecular Interactions in Biofilms. Chemistry & Biology 2002.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1558
    Illegal AgeI site found at 979
    Illegal AgeI site found at 1755
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


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Categories
Parameters
None